Viromics workshop

Viromics tutorial for the 9th EBAME workshop.

In this tutorial we will mine for phage sequences in a metagenome assembly.

Checking their quality, we can then select some of the predictions as candidate “Viral OTUs” (vOTUs).

When you have multiple samples, you can back-map the reads to the vOTUs to estimate their abundance in the original samples.

Using Anvi’o, we can generate a visualization focussed on the vOTUs, so building a CONTIGS.db database only based on the candidate vOTUs.

There are also some optional sections, covering how to automate and executing workflows written in NextFlow.

Tutorial developed by:

• Evelien Adriaenssens
• Andrea Telatin
• Alise Ponsero
• Ryan Cook
• Claire Elek
• Hannah Pye

from the Quadram Institute Bioscience, Norwich, UK.